An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
نویسندگان
چکیده
منابع مشابه
A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment.
Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires les...
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On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including standard methods for unsupported gels and new methods devised for thin (0....
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Silver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR-SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming ...
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and -IgM k and l light chains did not show the presence of monoclonal components. Reduction with mercaptoethanol (1:2 dilution), which dissociates complexes but does not dissociate albumin dimers, excluded the possible formation of complexes with a1-antitrypsin. Interferences by radio-opaque agents or medications, which could lead to the appearance in the CZE electropherograms of abnormal peaks...
متن کاملComparison of two silver staining techniques for detecting lipopolysaccharides in polyacrylamide gels.
The classic silver staining method for detecting bacterial lipopolysaccharides (LPSs) in polyacrylamide gels (C. Tsai and C. E. Frasch, Anal. Biochem. 119:115-119, 1982) was at least 20 times more sensitive than the modified silver staining method (A. Fomsgaard, M. A. Freudenberg, and C. Galanos, J. Clin. Microbiol. 28:2627-2631, 1990) for detecting LPS from the bacterium Moraxella osloensis. H...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 1990
ISSN: 0074-0276
DOI: 10.1590/s0074-02761990000100017